Multiplexing of up to samples per Nextera XT library is available for projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing.
Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers. These unique dual index codes use 10 bp codes. This change in base pair index codes requires adjustments to the sequencing run setup.
Find robotic systems compatible with this kit. This product is also available as an Illumina Advantage TG product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency. For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data.
This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use. The Nextera XT Library Preparation Kit eliminates the need for library quantification before sample pooling and sequencing.
Index Adapters Pooling Guide Documentation. Custom Protocol Selector Generates customized, end-to-end instructions. A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species. Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.
NGS-based microbial sequencing methods include shotgun metagenomics, 16S rRNA sequencing, whole-genome and de novo sequencing, and transcriptomics. Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever. With single-cell RNA-Seq, you can study cellular differences often masked by bulk sampling.
Explore high- and low-throughput single-cell analysis methods. The kit uses an efficient combination of high-performance magnetic beads and innovative silica-based chemistry.
High-performance magnetic particles ensure that the isolated DNA is of high yield and purity. The convenient MagAttract protocol allows reproducible results in as little as 70 minutes. Fast and optimized protocol Optimized buffers and enzymes gently lyse samples, while ensuring minimized fragmentation of genomic DNA.
The procedure comprises 4 simple steps: lyse, bind, wash, and elute. Following sample lysis, the DNA binds to the surface of magnetic beads. It can be used for the generation of libraries with both small and large inserts and is therefore suitable for all sequencing applications.
Supporting data and figures Successful isolation of high-molecular-weight DNA. PDF KB. Scientific Posters 1. Kit Handbooks 1. For isolation of high-molecular-weight DNA from blood, tissue, and bacteria for next-generation sequencing applications.
Quick-Start Protocols 1. PDF 73KB. Safety Data Sheets 1. Safety Data Sheets EN. Follow Us. Contact Us. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.
Find automation vendors that support the sample workflow. Karolinska Institutet researchers use Illumina sequencers to identify novel HPV types associated with non-melanoma skin cancers. Custom Protocol Selector Generates customized, end-to-end instructions.
Determine the best library prep kit or array for your needs based on your starting material and method of interest. A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species. Reagent kits for the NovaSeq System provide ready-to-use cartridge-based reagents for cluster generation and SBS.
This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome. Simple, all-inclusive whole-genome sequencing WGS library preparation that provides accurate and comprehensive coverage of complex genomes.
The NovaSeq Xp workflow enables labs to load libraries directly into each lane of a NovaSeq System flow cell, without additional instrumentation.
Cancer whole-genome sequencing provides researchers with a base-by-base view of the unique mutations and abnormalities present in cancer tissue.
Large whole-genome sequencing informs disease research and population genomics studies and reveals disease-associated alleles. Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits. Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.
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